Recombinant Cynomolgus CD47 Protein (Met1-Asn142), HlgG1 Fc-tagged

• Cat.No.: CD47-1017C
• Product Overview: Recombinant Cynomolgus CD47 Protein (XP_005548291.1) (Met1-Asn142) was produced by HEK293 Cells expression system. This protein was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Description: CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.
• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Predicted N Terminal: Gln 19
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant cynomolgus CD47 consists of 362 amino acids and predicts a molecular mass of 40.7 kDa.
• Protein length: Met1-Asn142
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: CD47 CD47 molecule [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: CD47
• Gene ID: 102139846
• mRNA Refseq: XM_005548234
• Protein Refseq: XP_005548291