Active Recombinant Rat B3gat1 Protein, His-tagged

• Cat.No.: B3gat1-798R
• Product Overview: Recombinant Rat B3gat1 (Accession # NP_446455) Asp75-Ile347, fused with an N-terminal human CD33 signal sequence and a 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Rat
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and DTT.
• Bio-activity: Measured by its ability to transfer GlcA from UDP-GlcA to lactose.The specific activity is >725 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Rat B3gat1, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
• Protein length: Asp75-Ile347
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: B3gat1 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Rattus norvegicus ]
• Official Symbol: B3gat1
• Synonyms: B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; glcAT-P; glcUAT-P; glucuronosyltransferase P; glucuronosyltransferase-P; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase; Hnk-1
• Gene ID: 117108
• mRNA Refseq: NM_054003
• Protein Refseq: NP_446455