Active Recombinant Porcine trypsin

• Cat.No.: trypsin-40P
• Product Overview: Our recombinant trypsin solution is formulated by aseptic filtration with porcine trypsin obtained from microbial fermentation as the main raw material. The product is produced under GMP regulations without adding any animal source raw materials, which prevents animal-derived virus contamination from the source. The solution has high biological safety and can completely substitute animal-derived trypsin. It can be widely used for the digestion of adherent cells. This product does not contain EDTA and has a pH of 7.4. pH: 7.3-7.5 Osmolarity: 270-320 mOsm/kg Mycoplasma assay: Negative Microbiological assay: Negative

Specification

• Species: Porcine
• Tag: Non
• Description: Trypsin is a serine protease endopeptidase secreted by the pancreas. Its recognition sites are lysine and arginine residues, with cleavage occurring at the carboxyl terminus of these residues. This product is recombinant porcine trypsin expressed in Pichia pastoris. It is manufactured under GMP-compliant conditions, contains no animal-derived components, and is free from animal-origin viral contaminants (e.g., swine influenza virus and porcine parvovirus). The preparation is inherently devoid of chymotrypsin activity. The activity is inhibited by serine protease inhibitors (e.g., PMSF, TLCK) and metal ion chelators (e.g., EDTA).
• Form: Solution
• Purity: > 95%
• Bio-activity: ≥ 150 USP U/mL
• Advantages: 1. High activity: 5800 USP U/mg or 17400 BAEE U/mg 2. Good digestion effect: Better cell viability, yield, and proliferation activity 3. Easy to use: With phenol red/EDTA or not/Customizable concentration or lyophilization
• Unit definition: One USP unit was defined as a 0.003 increase in absorption at 253 nm per minute of enzymatic digestion of BAEE in 3.2 mL of reaction system (1 cm optical path) at 25 centigrade, pH 7.6.
• Endotoxin: < 1.0 EU/mL
• Usage: Pre-warm recombinant trypsin solution and complete medium at 37 centigrade before use. Discard the supernatant of the culture medium. Wash the cells with an appropriate amount of DPBS, and then discard the DPBS. Add an appropriate amount of recombinant trypsin solution (0.5 ml per 10 cm^2) and incubate at 37 centigrade until the cells detach. Add 5-10 mL of pre-warmed complete medium, gently mix the cells, transfer the cell suspension to a sterile 15 ml centrifuge tube, and centrifuge at 100 g for 5-10 minutes. Discard the supernatant and add an appropriate amount of complete medium for cell culture.
• Applications: Dissociation of adherent cells
• Note: 1. Try to avoid freeze-thaw cycles of this product after receipt; 2. Please wear lab coat and disposable gloves when using; 3. This product should not be used directly for clinical diagnosis and treatment.
• Stability: This product should be stored at -20 centigrade and can be stored for at least 12 months. In addition, this product can be stored at 4 centigrade for 1-2 weeks.
• Storage: Store at -20 centigrade.
• Concentration: 0.025 mg/mL
• Shipping: Dry ice

Gene Information

• Official Symbol: Trypsin