Active Recombinant Porcine IL4

• Cat.No.: IL4-9291P
• Product Overview: Recombinant Porcine IL4 was expressed in CHO Cells.

Specification

• Species: Pig
• Source: CHO
• Tag: Non
• Description: Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50< 0.25="" ng/ml,="" measured="" in="" a="" cell="" proliferation="" assay="" using="" tf-1="" human="" erythroleukemic="" cells,="" corresponding="" to="" a="" specific="" activity="" of="">4 x 106 units/mg
• Molecular Mass: 15kDa, observed by reducing SDS-PAGE
• AA Sequence: His25-Cys133, expressed with an N-terminal Met.
• Endotoxin: <0.2 eu/μg,="" determined="" by="" lal="">
• Purity: >95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rpIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.

Gene Information

• Gene Name: IL4 interleukin 4 [ Sus scrofa (pig) ]
• Official Symbol: IL4
• Synonyms: IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1
• Gene ID: 397225
• mRNA Refseq: NM_214123
• Protein Refseq: NP_999288
• UniProt ID: Q04745
• Chromosome Location: 2; 2
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved bi