Active Recombinant Cynomolgus FCGRT/B2M protein, His-tagged

• Cat.No.: FCGRT&B2M-1537C
• Product Overview: Recombinant Cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Ser 297 & Met 1-Met 119
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind human IgG1 in functional ELISA.
• Molecular Mass: The recombinant heterodimer of cynomolgus FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa. The apparent molecular mass of cynomolgus FCGRT&B2M heterodimer is approximately 35 & 12 KDa respectively in SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.