Active Recombinant Pig CYP1A2 Protein

• Cat.No.: CYP1A2-132P
• Product Overview: Recombinant pig CYP1A2 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Biased expression in liver (RPKM 201.6) and mixtures (RPKM 79.4)
• Source: E. coli
• Species: Pig
• Bio-activity: Cytochrome c Reductase Activity: 145 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: 7-ethoxyresorufin O-deethylase
  Linear with time up to: 4 min(Determined at 0.2 µM substrate, using a P450 concentration of 1 pmol/ml)
  Linear with P450 up to: 10 pmol/ml(Determined at 0.2 µM substrate, using an incubation time of 30 sec)
  Vmax: 2.6 pmol/min/pmol CYP
  Km: 0.03 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 10.0 nmol/ml
  Protein Concentration: 19.3 mg/ml
  Specific P450 Content: 518 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP1A2 cytochrome P450, family 1, subfamily A, polypeptide 2 [ Sus scrofa (pig) ]
• Official Symbol: CYP1A2
• Synonyms: CYP1A2; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450 1A2
• Gene ID: 100152910
• mRNA Refseq: NM_001159614
• Protein Refseq: NP_001153086
• UniProt ID: F1SJ26