CYP1A2

  • Official Full Name

    cytochrome P450, family 1, subfamily A, polypeptide 2
  • Overview

    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
  • Synonyms

    CYP1A2;cytochrome P450, family 1, subfamily A, polypeptide 2;CP12;P3-450;P450(PA);cytochrome P450 1A2;P450 form 4;cytochrome P450 4;cytochrome P(3)450;cytochrome P450-P3;dioxin-inducible P3-450;microsomal monooxygenase;xenobiotic monooxygenase;aryl hydrocarbon hydroxylase;flavoprotein-linked monooxygenase;cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2

Recombinant Proteins

  • Human
  • Rat
  • Dog
  • Pig
  • Mouse
  • E.coli
  • Insect Cells
  • HEK293
  • Mammalian Cells
  • Wheat Germ
  • P.pastoris
  • In Vitro Cell Free System
  • Protein Conjugation
  • His
  • Non
  • DDK
  • Myc
  • GST
  • Avi
  • Fc
  • Flag
Cat.# Product name Source (Host) Species Tag Protein Length Price
CYP1A2-10H Recombinant Human CYP1A2 Protein, His-tagged E.coli Human His
CYP1A2-01H Active Recombinant Human CYP1A2 Protein E.coli Human Non
CYP1A2-02H Active Recombinant Human CYP1A2 Protein E.coli Human Non
CYP1A2-121H Recombinant Human CYP1A2 Insect Cells Human Non
CYP1A2-150H Recombinant Human CYP1A2, MYC/DDK-tagged HEK293 Human DDK&Myc
CYP1A2-1716R Recombinant Rat CYP1A2 Protein Mammalian Cells Rat His
CYP1A2-2245H Recombinant Human CYP1A2 Protein, GST-tagged Wheat Germ Human GST
CYP1A2-27559TH Recombinant Human CYP1A2 Wheat Germ Human Non 100 amino acids
CYP1A2-361H Recombinant Human CYP1A2/NADPH protein, Active P.pastoris Human Non
CYP1A2-551H Recombinant Human CYP1A2 Insect Cells Human Non
CYP1A2-7960H Recombinant Human CYP1A2 protein, His-tagged E.coli Human His Ala2~Ser231
Cyp1a2-7961R Recombinant Rat Cyp1a2 protein, His-tagged E.coli Rat His Val192~Glu444
CYP1A2-432HCL Recombinant Human CYP1A2 cell lysate Human Non
CYP1A2-119D Active Recombinant Dog CYP1A2 Protein E.coli Dog
CYP1A2-1297H Recombinant Human CYP1A2 Protein, His&GST-tagged E.coli Human GST&His Ala2-Ser231
CYP1A2-132P Active Recombinant Pig CYP1A2 Protein E.coli Pig
CYP1A2-1375R Recombinant Rat CYP1A2 Protein, His (Fc)-Avi-tagged HEK293 Rat Avi&Fc&His
CYP1A2-1375R-B Recombinant Rat CYP1A2 Protein Pre-coupled Magnetic Beads HEK293 Rat
Cyp1a2-141R Recombinant Rat CYP1A2 Protein E.coli Rat
CYP1A2-2304HF Recombinant Full Length Human CYP1A2 Protein, GST-tagged In Vitro Cell Free System Human GST Full L. 516 amino acids
Cyp1a2-2413M Recombinant Mouse Cyp1a2 Protein, Myc/DDK-tagged HEK293 Mouse DDK&Myc
CYP1A2-2796H Recombinant Human CYP1A2 Protein, His-tagged, OVA Conjugated Protein Conjugation Human His Thr284-Ser298
CYP1A2-68H Active Recombinant Human CYP1A2 Protein E.coli Human
CYP1A2-8756HFL Recombinant Full Length Human CYP1A2, Flag-tagged Mammalian Cells Human Flag Full L.
Kit-0924 CYP1A2 Blue Screening Kit Non
Kit-2126 Cytochrome P450 1A2 Fluorometric Activity Assay Kit Non
Kit-2127 Cytochrome P450 1A2 Inhibitor Screening Kit Non

    Background

    What is CYP1A2 Protein?

    CYP1A2 is a protein that works quietly but importantly in our bodies. Part of the cytochrome P450 family, it helps process a range of chemicals. You’ll mostly find CYP1A2 in the liver, where it breaks down things like drugs, caffeine, and some hormones. It acts like a natural filter, making sure we detoxify or convert what we consume into something useful or ready to be expelled. Because it plays a big part in drug metabolism, scientists are very interested in how CYP1A2 functions. When new medicines are being developed, understanding this protein is key because how active it is can change how well a drug works or what side effects might appear.

    Fig1. Body map of cytochrome P450 enzyme expression. (Sarah C Preissner, 2013)

    What is the Function of CYP1A2 Protein?

    CYP1A2 is a busy protein found in our liver and a part of the cytochrome P450 group. Its main job is to help break down all kinds of substances we consume. This includes processing medications, so they work effectively in our bodies. It also deals with caffeine and some hormones, ensuring they're handled the right way. You can think of CYP1A2 as a natural filter, detoxifying chemicals and turning them into forms we can either use or safely eliminate. How well CYP1A2 works can change how drugs behave in our system, affecting their success and any side effects. Because of this, scientists pay a lot of attention to CYP1A2, especially when creating new drugs, since variations in its activity can influence how well medicines work and interact.

    CYP1A2 Related Signaling Pathway

    CYP1A2 is part of an important signaling pathway in our body that involves breaking down various chemicals. Located mainly in the liver, this protein is key for processing drugs and compounds we encounter daily. It works by helping to modify these substances into something our bodies can use or get rid of. This pathway influences how effectively drugs work and their potential side effects. Changes in this signaling pathway can affect everything from how quickly a drug leaves our system to how active certain compounds become. Because of this, understanding the CYP1A2 pathway helps researchers and doctors predict drug interactions and personalize treatments for safer and more effective therapies.

    CYP1A2 Related Diseases

    CYP1A2 plays a big role in processing what we put into our bodies, and when it’s off, health issues can arise. It's especially important for how we break down drugs and caffeine. If CYP1A2 is out of whack, medicines might not perform right, or you might experience side effects since they're not broken down properly. Plus, research suggests that how active CYP1A2 is could affect the risk of some cancers, especially those linked to hormones, like breast cancer. Since CYP1A2 also helps detoxify substances, changes in its activity could impact liver-related conditions. Doctors keep a close eye on CYP1A2 to tailor treatments and potentially lower the risk of these issues by tracking how active this protein is.

    Bioapplications of CYP1A2

    CYP1A2 is a super important enzyme in our bodies, especially when it comes to handling different substances like medications and dietary elements. It's the one that helps process caffeine, for instance, and plays a part in how other drugs are broken down, such as some asthma medications. Beyond that, it also tackles some environmental toxins, helping to neutralize them. Factors like what you eat—take broccoli, for example—can actually ramp up its activity. Plus, researchers are digging into its potential connections to cancer, looking at how it might activate or deactivate certain carcinogens. This enzyme is really a testament to how our bodies manage so many intricate and crucial processes.

    Case Study

    Case Study 1: Zietek BM. et al. SLAS Discov. 2018

    By testing how drugs interact with cytochrome P450 enzymes like CYP1A2 early on, researchers can reduce failures in drug development. The nanofractionation platform helps with this. Here's how: a metabolic mix is split via liquid chromatography (LC), analyzed in tiny fractions on a plate using mass spectrometry (MS), and tested for CYP1A2 inhibition. This method creates a bioactivity map, identifying inhibitors by matching bioassay peaks with MS data. It successfully identified five CYP1A2 inhibitors and effectively tested mixtures with potent inhibitors and ellipticine metabolites, achieving clear separation and accurate bioactivity correlation.
    • Fig1. Detection of potent inhibitors of CYP1A2 present in a mixture.
    • Fig2. Detection of CYP1A2 inhibitors present in a Phase I metabolic mixture of ellipticine.

    Case Study 2: Hou C. et al. BMC Cancer. 2024

    Circular RNA, or circRNAs, are important in colorectal cancer (CRC) development. However, the role of circ_0008345, transcribed by PTK2, isn't fully understood. In this study, researchers explored what circ_0008345 does in CRC. They confirmed circ_0008345's circular structure and found it mainly in the cytoplasm. It binds to miR-182-5p, which targets the CRC oncogene CYP1A2. Drop in circ_0008345 lowered colony formation, cell movement, and tumor growth in mice. However, these effects were reversed when miR-182-5p was also knocked down. They discovered that METTL3 modifies circ_0008345 with m6A, and lowering METTL3 drops circ_0008345 levels.
    • Fig3. The CYP1A2 expression in FHC, DLD1, and SW480 cells using immunoblotting.
    • Fig4. The mRNA expression of CYP1A2 in response to different infections by RT-qPCR.

    Quality Guarantee

    High Purity

    Involved Pathway

    CYP1A2 involved in several pathways and played different roles in them. We selected most pathways CYP1A2 participated on our site, such as Aflatoxin B1 metabolism,Aflatoxin activation and detoxification,AhR pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with CYP1A2 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

    Pathway Name Pathway Related Protein
    Caffeine metabolism NAT,XDH,CYP2A6,PNAT10,Nat3,NAT1,UOX,PNAT3,NAT2
    Aflatoxin B1 metabolism PRKRA,CYP3A4,CYP2A13,FAM84B
    AhR pathway JUND,CDKN1B,CDKN1A,CYP1B1,CYP1A1,AIP,FLG,JUN,PTGES3,EGFR
    Arachidonic acid metabolism CYP4A14,GPX5,CYP2B19,MAPKAPK2B,CYP2AD2,PLA2G2E,FAAH2A,PLA2G4B,PTGR1,GPX6
    Aflatoxin activation and detoxification ACY3.1,DPEP2,FAM84B,CYP3C1,CYP2A13,ACY3,CYP3A4,CYP3C4,GGT6,GGTLC1
    Arylamine metabolism NAT2
    Biological oxidations CYP2K17,CYP4A11,GLYATL1,Sult2a6,CYP2S1,ABHD14B,CYP11B1,CYP2A13,UGT1B4,UGT1B3

    • Fig1. Main pathways and enzymes involved in caffeine degradation. (Jingjing Yu, 2024)
    • Fig2. Two-step metabolic activation of clopidogrel within the liver. (Frank A Plakogiannis, 2024)

    Protein Function

    CYP1A2 has several biochemical functions, for example, aromatase activity,caffeine oxidase activity,demethylase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CYP1A2 itself. We selected most functions CYP1A2 had, and list some proteins which have the same functions with CYP1A2. You can find most of the proteins on our site.

    Function Related Protein
    aromatase activity CYP2D10,CYP1A4,CYP2U1,CYP2C37,CYP2J6,CYP3A5,CYP2A13,CYP3A25,CYP2C9,CYP2A12
    caffeine oxidase activity CYP3A4,CYP2C38,FAM84B,CYP2C9,CYP2C39
    heme binding CYGB,SDHDA,CYP2K19,PTGIS,CYP26A1,FAM84B,CYP2AD2,CYP2AA1,CYP4F11,CYCS
    electron carrier activity NDUFA12,GRXCR1,AKR7A2,COX11,NQO2,FDX1,GRX2,AOX3L1,NDUFS1,LOXL2
    oxidoreductase activity CYP4F18,FDFT1,DHRS11A,CYP2X9,CYP2K19,AIFM4,CYP2C19,SDR16C5A,HMGCRA,GPD1A
    iron ion binding ALKBH8,CYP2K18,CYGB1,OGFOD2,CYP2AA9,ALOX5,CYP4A10,MSMO1,FDX1,CYP2R1
    enzyme binding PHKG2,MLEC,SCNM1-PS,PIAS3,CCND1,APOA1,NFKBIA,PRKCA,YWHAB,KMT2E
    demethylase activity CYP3A41B,ALKBH4,CYP3A41A,CYP1A1,KDM1A
    monooxygenase activity CYP1A,CYP51,CYP17A2,CYP2AA3,CYP46A1.2,CYP2C39,CYP2Y3,CYP2P6,CYP2F1,CYP4V7

    Interacting Protein

    CYP1A2 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CYP1A2 here. Most of them are supplied by our site. Hope this information will be useful for your research of CYP1A2.

    Resources

    References

    • von der Heyde, S; Wagner, S; et al. mRNA Profiling Reveals Determinants of Trastuzumab Efficiency in HER2-Positive Breast Cancer. PLOS ONE 10:-(2015).
    • Czerwinski, M; Kazmi, F; et al. Anti-CD28 Monoclonal Antibody-Stimulated Cytokines Released from Blood Suppress CYP1A2, CYP2B6, and CYP3A4 in Human Hepatocytes In Vitro. DRUG METABOLISM AND DISPOSITION 43:42-52(2015).

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