Active Recombinant Human TNFSF9

• Cat.No.: 4-1BB-L-516H
• Product Overview: Recombinant Human 4-1BB-L produced in E. coli is approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: 4-1BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 4-1BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has been shown to interact with TRAF2. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD). The human 4-1BBL ECD shares 32 % and 35 % a.a. identity with murine and rat ECD.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by stimulation of IL-8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of >1.0 × 10^5 IU/mg.
• Molecular Mass: 19.4 kDa
• AA Sequence: REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE
• Endotoxin: Less than 1 EU/μg of rHu4-1BBL as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apport
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportion

Gene Information

• Gene Name: TNFSF9 tumor necrosis factor (ligand) superfamily, member 9 [ Homo sapiens (human) ]
• Official Symbol: 4-1BB-L
• Synonyms: TNFSF9;CD137L; 4-1BB-L;tumor necrosis factor ligand superfamily member 9;4-1BBL;4-1BB ligand;receptor 4-1BB ligand;homolog of mouse 4-1BB-L
• Gene ID: 8744
• mRNA Refseq: NM_003811.3
• Protein Refseq: NP_003802.1
• MIM: 606182
• UniProt ID: P41273
• Chromosome Location: 19p13.3
• Pathway: Cytokine-cytokine receptor interaction
• Function: cytokine activity;receptor binding;tumor necrosis factor receptor binding