Active Recombinant Mouse Tnfsf11 Protein, His-tagged

• Cat.No.: Tnfsf11-953M
• Product Overview: Recombinant Mouse Tnfsf11 (Accession # O35235) Arg72-Asp316, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells.The ED50 for this effect is typically 5-15 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody. An E. coli-expressed Recombinant Mouse (rm) TRANCE (aa 158-317) is also available. The ED50 for the E. coli-expressed rmTRANCE is 0.5-2 ng/mL.
• Molecular Mass: Recombinant Mouse Tnfsf11, His-tagged has a calculated MW of 28 kDa. In SDS-PAGE migrates as 36 kDa, reducing conditions.
• Protein length: Arg72-Asp316
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Tnfsf11 tumor necrosis factor (ligand) superfamily, member 11 [ Mus musculus ]
• Official Symbol: Tnfsf11
• Synonyms: TNFSF11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11; OPG ligand; osteoprotegerin ligand; osteoclast differentiation factor; receptor activator of NF-kappaB ligand; TNF-related activation-induced cytokine; receptor activator of nuclear factor kappa B ligand; receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor-related activation-induced cytokine; ODF; OPG; OPGL; RANKL; Ly109l; Trance
• Gene ID: 21943
• mRNA Refseq: NM_011613
• Protein Refseq: NP_035743