Active Recombinant Mouse TNFa Protein, Met-tagged

• Cat.No.: TNFa-731M
• Product Overview: Recombinant Mouse TNFa Protein(NP_038721.1) (Leu 80-Leu 235) was expressed in E. coli, with an initial Met at the N-terminus.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Leu 80-Leu 235
• Form: Lyophilized from sterile PBS, pH7.4
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
• Molecular Mass: The recombinant mouse TNF-α consists of 157 amino acids and migrates with an apparent molecular mass of 17.39 kDa as estimated in SDS-PAGE under reducing conditions.
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.