Active Recombinant Mouse Tigit, Fc-tagged

• Cat.No.: Tigit-108M
• Product Overview: A DNA sequence encoding the mouse TIGIT (PP86176) (Met1-Gly141) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: Human Cells
• Tag: Fc
• Protein Length: 1-141 a.a.
• Description: TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
• Predicted N Terminal: Gly 26
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse PVR-His at 10 μg/ml (100 μl/well) can bind mouse TIGIT-Fc, The EC50 of mouse TIGIT-Fc is 0.25-0.55 μg/ml.
• Molecular Mass: The recombinant mouse TIGIT /Fc is a disulfide-linked homodimer. The reduced monomer comprises 357 amino acids and has a predicted molecular mass of 39.8 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC
• Storage: Store it under sterile conditions at -20ºC to -80ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Tigit T cell immunoreceptor with Ig and ITIM domains [ Mus musculus (house mouse) ]
• Official Symbol: Tigit
• Synonyms: Tigit; T cell immunoreceptor with Ig and ITIM domains; Vstm3; ENSMUSG00000071552; T-cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; tigit
• Gene ID: 100043314
• mRNA Refseq: NM_001146325
• Protein Refseq: NP_001139797
• UniProt ID: P86176
• Chromosome Location: 16; 16 B4
• Pathway: Cell adhesion molecules (CAMs)
• Function: receptor binding