Active Recombinant Mouse ST2 protein(Met1-Arg332), hFc-tagged

• Cat.No.: ST2-1622M
• Product Overview: Recombinant Mouse ST2 (NP_001020773.1) (Met1-Arg332) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Protein length: Met1-Arg332
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. 2. Immobilized Canine IL33 at 10μg/mL (100μL/well) can bind mouse IL1RL1-Fc, the EC50 of mouse IL1RL1-Fc is 0.05-0.3 μg/mL.
• Molecular Mass: The recombinant mouse ST2 comprises 544 amino acids and has a predicted molecular mass of 61.8 kDa. The apparent molecular mass of the protein is approximately 93.2 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.