Active Recombinant Mouse St14 Protein, Met & His-tagged

• Cat.No.: St14-653M
• Product Overview: Recombinant Mouse St14 (Accession # NP_035306) Gly596-Val855, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: Met & Val615
• Form: Supplied as a 0.2 µm filtered solution in Tris and Glycerol.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC.The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse St14, Met & His-tagged has a calculated MW of 3 kDa & 26 kDa. In SDS-PAGE migrates as 28 kDa, reducing conditions.
• Protein length: Gly596-Val855
• Purity: >75%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: St14 suppression of tumorigenicity 14 (colon carcinoma) [ Mus musculus ]
• Official Symbol: St14
• Synonyms: ST14; suppression of tumorigenicity 14 (colon carcinoma); suppressor of tumorigenicity 14 protein homolog; matriptase/MT-SP1; serine protease 14; protease, serine, 14 (epithin); mCAP3; MT-SP1; Prss14; Epithin; Tmprss14; matriptase
• Gene ID: 19143
• mRNA Refseq: NM_011176
• Protein Refseq: NP_035306