Active Recombinant Mouse Spint2, His-tagged

• Cat.No.: Spint2-671M
• Product Overview: Recombinant Mouse Spint2 (Accession # NP_001076017) Arg30-Lys140, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 30-140 a.a.
• Predicted N Terminal: Arg30
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 .The IC50 value is <2 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Spint2, His-tagged has a calculated MW of 14 kDa. In SDS-PAGE migrates as 23 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Spint2 serine protease inhibitor, Kunitz type 2 [ Mus musculus ]
• Official Symbol: Spint2
• Synonyms: SPINT2; serine protease inhibitor, Kunitz type 2; kunitz-type protease inhibitor 2; hepatocyte growth factor activator inhibitor type 2; HAI-2; C76321; AL024025
• Gene ID: 20733
• mRNA Refseq: NM_001082548
• Protein Refseq: NP_001076017