Active Recombinant Mouse Slit3 Protein, His-tagged

• Cat.No.: Slit3-123M
• Product Overview: Recombinant Mouse Slit3 Protein (Cys34-Val1116), with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: Predicted to enable Roundabout binding activity and heparin binding activity. Involved in aortic valve morphogenesis; atrioventricular valve morphogenesis; and ventricular septum morphogenesis. Acts upstream of or within several processes, including axon guidance; negative regulation of cell population proliferation; and negative regulation of gene expression. Located in extracellular space. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; heart; and sensory organ. Used to study congenital diaphragmatic hernia. Orthologous to human SLIT3 (slit guidance ligand 3).
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: His
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit3, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
• Molecular Mass: Predicted Molecular Mass: 122 kDa
  SDS-PAGE: 121-153 kDa, reducing conditions
• N-terminal Sequence Analysis: Cys34
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Ethylene Glycol.
• Shipping: The product is shipped with dry ice or equivalent.
• Protein length: 34-1116 a.a.

Gene Information

• Gene Name: Slit3 slit guidance ligand 3 [ Mus musculus (house mouse) ]
• Official Symbol: Slit3
• Synonyms: Slit3; slit guidance ligand 3; Slil2; Slit1; b2b2362.1Clo; slit homolog 3 protein; slit-3
• Gene ID: 20564
• mRNA Refseq: NM_011412
• Protein Refseq: NP_035542
• UniProt ID: Q9WVB4