Active Recombinant Mouse Sema6a Protein, Fc-tagged

• Cat.No.: SEMA6A-14874M
• Product Overview: Recombinant Mouse Sema 6A (Gly19-Thr649) Protein with Human IgG1 (Pro100-Lys330) tag was expressed in Spodoptera frugiperda, Sf 21.

Specification

• Description: Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation.
• Source: Insect cell
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Gly19
• Bio-activity: Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 0.2-1.2 μg/mL.
• Molecular Mass: 97 kDa
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 19-649 a.a.

Gene Information

• Gene Name: Sema6a sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A [ Mus musculus (house mouse) ]
• Official Symbol: Sema6a
• Synonyms: Sema6a; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; se; Sem; VIa; Sema6; Semaq; b2b997C; AI851735; Sema6A-1; A730020P05Rik; semaphorin-6A; Mutant line 997; sema Q; sema VIA; semaphorin Q; semaphorin VIA; semaphorin-6A-1
• Gene ID: 20358
• mRNA Refseq: NM_018744
• Protein Refseq: NP_061214
• UniProt ID: O35464