Active Recombinant Mouse SELP protein, His-tagged

• Cat.No.: SELP-305M
• Product Overview: Recombinant Mouse SELP protein(Q01102)(Met1-Ala709), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-709 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >60% cells will adhere specifically after 60 minutes at 37℃.
• Molecular Mass: The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Ala709
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Selp selectin, platelet [ Mus musculus ]
• Official Symbol: SELP
• Synonyms: SELP; selectin, platelet; P-selectin; granule membrane protein 140; CD62 antigen-like family member P; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; Grmp; CD62P; LECAM3; PADGEM; GMP-140; MGC129336; MGC129337
• Gene ID: 20344
• mRNA Refseq: NM_011347
• Protein Refseq: NP_035477