Active Recombinant Mouse Rln1

• Cat.No.: Rln1-656M
• Product Overview: Recombinant Mouse Rln1 (Accession # NP_035402) Arg23-Arg54, fused with an N-terminal Met (B chain) & Glu161-Cys185 (A chain), was produced in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Arg23-Arg54
• Predicted N Terminal: Met (B chain) & Glu161 (A chain)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce cAMP accumulation in THP-1 human acute monocytic leukemia cells. Parsell, D.A. et al. (1996) J. Biol. Chem. 271:27936.The ED50 for this effect is typically 2 - 8 ng/mL.
• Molecular Mass: Recombinant Mouse Rln1, Met-tagged has a calculated MW of 3.9 kDa (B chain) & 2.7 kDa (A chain). In SDS-PAGE migrates as 4 & 3 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 250 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Rln1 relaxin 1 [ Mus musculus ]
• Official Symbol: Rln1
• Synonyms: RLN1; relaxin 1; prorelaxin 1; Rln; rlx
• Gene ID: 19773
• mRNA Refseq: NM_011272
• Protein Refseq: NP_035402