Active Recombinant Mouse RIKEN CDNA 2610109H07 Gene, His-tagged

• Cat.No.: 2610109H07Rik-053M
• Product Overview: Recombinant Mouse 2610109H07Rikprotein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Gly26-Ile343.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• ProteinLength: 26-343 a.a.
• Description: 2610109H07Rik alsocalled neucrin (neural tissuespecific cysteine-rich protein) is a secreted,58 kDa, presumably glycosylated member of the draxin family of repulsiveguidance proteins. In mammals, it is expressed in developing neurons (axons),astroglia, and likely cells of the developing somite. Its expression appearsto be limited to the brain and spinal cord. Mouse 2610109H07Rik mRNA encodes343 amino acids (aa) that include a 25 aa signal sequence and a 318 aamature, secreted protein containing one potential N-linked glycosylation sitefollowed by a Cys-rich domain (aa 268-327).
• Form: Lyophilized from a0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Gly26
• Molecular Weight: 36.4 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When recombinant mouse 2610109H07Rik is immobilized at 2 μg/mL, recombinant human LRP-6 FcChimera binds with an apparent KD < 20 nM.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 45-60kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: 2610109H07Rik RIKEN cDNA2610109H07 gene [ Mus musculus ]
• Official Symbol: 2610109H07Rik
• Synonyms: 2610109H07Rik;RIKEN cDNA 2610109H07 gene; Draxin; neucrin; dorsal repulsive axon guidanceprotein
• Gene ID: 70433
• mRNA Refseq: NM_027426
• Protein Refseq: NP_081702
• UniProt ID: Q6PAL1
• Chromosome Location: 4; 4 E1
• Function: protein binding