Active Recombinant Mouse PVR Protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: Pvr-624M
• Product Overview: Biotinylated Mouse CD155, Fc Tag, Avi Tag is expressed from HEK293 cells. It contains AA Asp 29 - Leu 348 (Accession # Q8K094-1). This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc&Avi
• Predicted N Terminal: Asp 29
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Mouse TIGIT, Fc Tag at 5 ug/mL (100 uL/well) can bind Biotinylated Mouse CD155, Fc Tag, Avi Tag with a linear range of 20-313 ug/mL.
• Molecular Mass: The protein has a calculated MW of 63.5 kDa. The protein migrates as 90-120 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 29-348 a.a.

Gene Information

• Gene Name: Pvr poliovirus receptor [ Mus musculus ]
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; PVS; mE4; HVED; Taa1; CD155; Tage4
• Gene ID: 21332