Active Recombinant Mouse Prss32 Protein, His-tagged

• Cat.No.: Prss32-760M
• Product Overview: Recombinant Mouse Prss32 (Accession # NP_081496) Ser20-Arg304, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser20
• Form: Lyophilized from a 0.2 µm filtered solution in MES and NaCl.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Arg-ThioBenzyl ester (Z-R-SBzl), in the presence of 5,5"Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.The specific activity is >400 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Prss32, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 40 kDa doublet, reducing conditions.
• Protein length: Ser20-Arg304
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Prss32 protease, serine 32 [ Mus musculus (house mouse) ]
• Official Symbol: Prss32
• Synonyms: mT5; tryptase-5; 2010001P08Rik; protease, serine, 32; protease, serine, 32; eosinophil serine protease; testisin; tryptase 5
• Gene ID: 69814
• mRNA Refseq: NM_027220.2
• Protein Refseq: NP_081496.2
• Chromosome Location: 17 A3.3; 17 12.09 cM