Active Recombinant Mouse Pkm Protein (1-531aa), N-6×His-tagged

• Cat.No.: Pkm-13M
• Product Overview: Recombinant mouse Pkm protein (1-531aa) fused to His-tag at Nterminus was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 1-531
• Description: Enables mRNA binding activity and pyruvate kinase activity. Involved in positive regulation of cytoplasmic translation. Acts upstream of or within glycolytic process. Located in cilium; photoreceptor inner segment; and rough endoplasmic reticulum. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Orthologous to human PKM (pyruvate kinase M1/2).
• Form: Liquid
• Bio-activity: Specific activity: > 50,000 pmol/min/μg. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37 centigrade.
• Molecular Mass: 60.2 kDa (554aa) Confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSMPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
• Purity: >85% by SDS-PAGE
• Applications: Enzyme Activity, SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: 20mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol

Gene Information

• Gene Name: Pkm pyruvate kinase, muscle [ Mus musculus (house mouse) ]
• Official Symbol: Pkm
• Synonyms: Pkm; pyruvate kinase, muscle; Pk3; Pk-2; Pk-3; Pkm2
• Gene ID: 18746
• mRNA Refseq: NM_011099
• Protein Refseq: NP_035229
• UniProt ID: P52480