Active Recombinant Mouse PDGFA Protein

Description :	Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
Source :	E. coli
Species :	Mouse
Bio-activity :	3T3 cell proliferation, ≤10 ng/mL
Molecular Mass :	Dimer, 14.5/29.0 kDa (126/252 aa)
AA Sequence :	MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLNPDHREEETGRRRESGKNRKRKRLKPT
Endotoxin :	≤1 EUs/μg, Kinetic LAL
Purity :	≥95%, Reducing and Non-Reducing SDS PAGE
Stability :	12 months from date of receipt when stored at -20 to -80 centigrade as supplied. 1 month when stored at 4 centigrade after reconstituting as directed. 3 months when stored at -20 to -80 centigrade after reconstituting as directed.
Storage :	Storage Prior to Reconstitution: -20 centigrade
Storage Buffer :	Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Reconstitution :	Sterile water at 0.1 mg/mL
Shipping :	Room temperature
Instructions :	Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Name	Pdgfa platelet derived growth factor, alpha [ Mus musculus (house mouse) ]
Official Symbol	PDGFA
Synonyms	PDGFA; platelet derived growth factor, alpha; platelet-derived growth factor subunit A; PDGF-1; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide;
Gene ID	18590
mRNA Refseq	NM_008808
Protein Refseq	NP_032834
UniProt ID	P20033