Active Recombinant Mouse NCR1 protein, His-tagged

• Cat.No.: NCR1-10489M
• Product Overview: Recombinant Mouse NCR1 (NP_034876.2) (Met1-Asn255) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Asn255
• Description: NCR1, also known as NK-p46 and CD335, is a natural cytotoxicity receptor(NCR). NCRs are type I transmembrane proteins with 1-2 extracellular immunoglobulin domains, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. All are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. NKp46 has two extracellular Ig-like domains followed by a ~4 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells, pathogen-infected cell lines and mononuclear phagocytes infected with an intracellular bacterium.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind with recombinant human CD247/Biotin in a functional ELISA.
• Molecular Mass: The recombinant mouse NCR1 consists of 250 amino acids and predicts a molecular mass of 28.6 kDa.
• Endotoxin: <1.0 EU per μg protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: NCR1
• Official Symbol: NCR1
• Synonyms: Ly94 Protein, Mouse; NKp46 Protein, Mouse
• Gene ID: 17086
• mRNA Refseq: NM_010746.3
• Protein Refseq: NP_034876.2
• UniProt ID: Q8C567