Active Recombinant Human NCR1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: NCR1-0524H
• Product Overview: Biotinylated Recombinant Human NCR1 protein(Gln22-Asn254), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 22-254 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Anti-NKP46 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human NKp46, Fc,Avitag, premium grade with a linear range of 0.1-8 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 54.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln22-Asn254
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NCR1 natural cytotoxicity triggering receptor 1 [ Homo sapiens ]
• Official Symbol: NCR1
• Synonyms: NCR1; natural cytotoxicity triggering receptor 1; LY94, lymphocyte antigen 94 (mouse) homolog (activating NK receptor; NK p46); CD335; NK p46; NKP46; NK cell-activating receptor; natural killer cell p46-related protein; lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); LY94; NK-p46; FLJ99094
• Gene ID: 9437
• mRNA Refseq: NM_001145457
• Protein Refseq: NP_001138929
• MIM: 604530
• UniProt ID: O76036