Active Recombinant Mouse Ncam1, His-tagged

• Cat.No.: Ncam1-685M
• Product Overview: Recombinant Mouse Ncam1 (Accession # NP_001106675) Met1-Thr711, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Leu20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When 5 x 10^4 cells/well are added to mouse NCAM-1 coated plates (25 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Ncam1, His-tagged has a calculated MW of 77.4 kDa. In SDS-PAGE migrates as 105-140 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 1-711 a.a.

Gene Information

• Gene Name: Ncam1 neural cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Ncam1
• Synonyms: NCAM1; neural cell adhesion molecule 1; N-CAM-1; CD56; Ncam; E-NCAM; NCAM-1
• Gene ID: 17967
• mRNA Refseq: NM_001081445
• Protein Refseq: NP_001074914