Active Recombinant Mouse MSLN protein, His-Avi-tagged, Biotinylated

• Cat.No.: MSLN-0384M
• Product Overview: Biotinylated Recombinant Mouse MSLN protein(Asp298-Ser600), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His&Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Mouse Mesothelin, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human MSLN Antibody, Human IgG1 with a linear range of 10-78 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 37.7 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asp298-Ser600
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 298-600 a.a.

Gene Information

• Gene Name: Msln mesothelin [ Mus musculus ]
• Official Symbol: MSLN
• Synonyms: MSLN; mesothelin; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; MPF
• Gene ID: 56047
• mRNA Refseq: NM_018857
• Protein Refseq: NP_061345