Active Recombinant Mouse Met Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Met-730MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Metprotein was expressed in Murine myeloma cell line with the Fc part of MouseIgG2A Glu98-Lys330 (C-terminus) and Met1-Asn929 (N-terminus).

Specification

• Description: Met is aglycosylated receptor tyrosine kinase that plays a central role in epithelialmorphogenesis and cancer development. Met is synthesized as a single chainprecursor which undergoes cotranslational proteolytic cleavage. This generatesa mature Met that is a disulfide-linked dimer composed of a 50 kDaextracellular α chain and a 145 kDa transmembrane β chain.
• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Measuredby its ability to bind recombinant mouse Met in a functional ELISA with anestimated KD < 0.2 nM.
• Molecular Mass: 96 & 32 kDa; SDS-PAGE: 110-120 kDa & 40-43 kDa, reducing conditions.
• N-terminal Sequence Analysis: Glu 25 & Ser 307
• Endotoxin: < 1.0 EU/ μg of the protein by the LAL method.
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditionsafter reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Concentration: 200 μg/mL
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: 200 μg/mL in PBS
• Protein length: 1-929 a.a.

Gene Information

• Gene Name: Met met proto-oncogene [ Musmusculus ]
• Official Symbol: Met
• Synonyms: Met; metproto-oncogene; HGF; HGFR; Par4; c-Met; AI838057; hepatocyte growth factorreceptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogenetyrosine kinase; EC 2.7.10.1; Met protein
• Gene ID: 17295
• mRNA Refseq: NM_008591
• Protein Refseq: NP_032617
• UniProt ID: P16056