Active Recombinant Mouse Mep1a protein, His-tagged

• Cat.No.: Mep1a-2300M
• Product Overview: Recombinant Mouse Mep1a protein(NP_032611.2)(Met1-Arg615), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-615 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH.The specific activity is >400 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Trypsin for an activated form)
• Molecular Mass: The recombinant mouse MEP1A consists of 606 amino acids after removal of the signal peptide and has a predicted molecular mass of 69 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm MEP1A is approximately 80-90 kDa due to glycosylation.
• Proteinlength: Met1-Arg615
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Mep1a meprin 1 alpha [ Mus musculus ]
• Official Symbol: Mep1a
• Synonyms: MEP1A; meprin 1 alpha; meprin A subunit alpha; meprin alpha; endopeptidase-2; meprin A alpha-subunit; Mep1; Mep-1; Mep-1a; AI098089; AW107200
• Gene ID: 17287
• mRNA Refseq: NM_008585
• Protein Refseq: NP_032611