Active Recombinant Mouse LIFR protein(Met1-Ser828), His-tagged

• Cat.No.: LIFR-868M
• Product Overview: Recombinant Mouse LIFR (NP_038612.1) (Met 1-Ser 828) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser828
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind mouse LIF-Fc in a functional ELISA. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 5-20 ng/mL in the presence of 2 ng/mL recombinant human LIF.
• Molecular Mass: The recombinant mouse LIFR consists of 796 amino acids and has a predicted molecular mass of 90 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm LIFR is approximately 110-120 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lifr leukemia inhibitory factor receptor [ Mus musculus ]
• Official Symbol: LIFR
• Synonyms: LIFR; leukemia inhibitory factor receptor; LIF-R; LIF receptor; D-factor/LIF receptor; leukemia inhibitory factor receptor alpha chain; soluble differentiation-stimulating factor receptor; LIF; AW061234; A230075M04Rik
• Gene ID: 16880
• mRNA Refseq: NM_001113386
• Protein Refseq: NP_001106857