Active Recombinant Mouse Lif protein, His-tagged

• Cat.No.: Lif-757M
• Product Overview: Recombinant Mouse Lif protein(P09056)(Met1-Phe203), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-203 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.The ED50 for this effect is typically 0.1-0.5 ng/ml.
• Molecular Mass: The secreted recombinant mouse LIF comprises 191 amino acids and has a calculated molecular mass of 21.3 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 35-45 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Phe203
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus ]
• Official Symbol: Lif
• Synonyms: LIF; leukemia inhibitory factor; d factor; differentiation-stimulating factor
• Gene ID: 16878
• mRNA Refseq: NM_001039537
• Protein Refseq: NP_001034626