Active Recombinant Human LIF protein, His-tagged

• Cat.No.: LIF-134H
• Product Overview: Recombinant Human LIF(23-202aa) fused with His tag at C-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 23-202 a.a.
• Description: LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects is less or equal to 0.5 ng/ml.
• Molecular Mass: 20.8kDa (189aa)
  18-40kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: ADPSPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA
  QAFHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: > 90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: LIF leukemia inhibitory factor [ Homo sapiens ]
• Official Symbol: LIF
• Synonyms: LIF; leukemia inhibitory factor; CDF; cholinergic differentiation factor; DIA; differentiation inhibitory activity; differentiation inducing factor; hepatocyte stimulating factor III; HILDA; human interleukin in DA cells; D factor; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; differentiation-stimulating factor; MLPLI
• Gene ID: 3976
• mRNA Refseq: NM_002309
• Protein Refseq: NP_002300
• MIM: 159540
• UniProt ID: P15018
• Chromosome Location: 22q12.2
• Pathway: Adipogenesis, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Direct p53 effectors, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem
• Function: cytokine activity; growth factor activity; leukemia inhibitory factor receptor binding; leukemia inhibitory factor receptor binding; receptor binding