Active Recombinant Mouse Lif Protein (Animal Free-Ready-to-Use, 181 amino acid)

• Cat.No.: Lif-32M
• Product Overview: Recombinant Mouse Lif Protein (Animal Free-Ready-to-Use) without tag was expressed in E. coli. The protein is manufactured using all non-animal reagents.

Specification

• Species: Mouse
• Source: E.coli
• ProteinLength: 181 amino acid
• Description: Enables cytokine activity and growth factor activity. Acts upstream of or within several processes, including animal organ development; generation of neurons; and positive regulation of macromolecule metabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; cranium; early conceptus; and female reproductive system. Orthologous to human LIF (LIF interleukin 6 family cytokine).
• Bio-activity: Mouse LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 × 10^6 units/ml and 1 × 10^7 units/ml, where 50 units is defined as the amount of mouse LIF required to induce differentiation in 50% of the M1 colonies in 1 mL of medium.
• Molecular Mass: 20 kDa
• Endotoxin: < 0.01 ng/μg cytokine as determined by the LAL assay
• Purity: > 98% by SDS-PAGE
• Usage: 1 ml of mouse LIF with the specific activity of 1x106 units/ml is sufficient to treat 1 L of embryonic stem cell culture media. 1 mL of mouse LIF with the specific activity of 1x107 units/ml is sufficient to treat 10 L of embryonic stem cell culture media.
• Storage: It is stable for 6 to 12 months at 2-4 centigrade in original formulation. For extended storage, it is recommended to store in working aliquots at -20 to -80 centigrade. Freeze-thaw should be avoided as it can result in loss of activity.
• Storage Buffer: 1×phosphate-buffered saline with 0.02% Tween-20. Sterile filtered through a 0.2 micron filter.
• Shipping: This product is shipped at ambient temperature with cold pack.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Mus musculus (house mouse) ]
• Official Symbol: Lif
• Synonyms: LIF; leukemia inhibitory factor; d factor; differentiation-stimulating factor
• Gene ID: 16878
• mRNA Refseq: NM_001039537
• Protein Refseq: NP_001034626
• UniProt ID: P09056