Active Recombinant Mouse Interleukin 5 Receptor, Alpha, Fc-tagged

• Cat.No.: Il5ra-5663M
• Product Overview: RecombinantMouse Il5ra protein was expressed in Murine myeloma celllinewith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl5ra Asp18-His339 (N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 18-339 a.a.
• Description: Il5ra isa 60 kDa hematopoietin receptor that plays a dominant role in eosinophilbiology. Mature mouse Il5ra consists of a 322 amino acid (aa) extracellular domain(ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment,and a 54 aa cytoplasmic domain. Within the ECD, mouse Il5ra shares 71% and 86%aa sequence identity with human and rat Il5ra, respectively. Alternatesplicing of mouse Il5ra generates soluble secreted forms which function asIL-5 antagonists.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: Asp18
• MolecularWeight: 63.2 kDa (monomer)
• Activity: Measuredby its binding ability in a functional ELISA. When 0.5 μg/mL of RecombinantMouse Il5ra/CD125 FcChimera is immobilized onto a goat anti-human IgG Fc antibody coated plate,the concentration of biotinylated recombinant mouse IL-5 that produces 50% ofthe optimal binding response is found to be approximately 4-16 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 75-85kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il5ra interleukin 5 receptor,alpha [ Mus musculus ]
• Official Symbol: Il5ra
• Synonyms: Il5ra; interleukin 5receptor, alpha; Il5r; CD125; CDw125; interleukin-5 receptor subunit alpha;IL-5RA; IL-5R-alpha; IL-5R subunit alpha; IL-5 receptor alpha chain; IL-5receptor subunit alpha
• Gene ID: 16192
• mRNA Refseq: NM_008370
• Protein Refseq: NP_032396
• UniProt ID: P21183
• Chromosome Location: 6
• Pathway: Cytokine Signalingin Immune system; Cytokine-cytokine receptor interaction; Hematopoietic celllineage; IL-5 Signaling Pathway; Immune System; Inflammatory Response Pathway;Interleukin receptor SHC signaling; Interleukin-2 signaling; Interleukin-3, 5and GM-CSF signaling; Jak-STAT signaling pathway; Signaling by Interleukins
• Function: cytokine receptoractivity; receptor activity