Active Recombinant Mouse Interleukin 15, His-tagged

• Cat.No.: Il15-533M
• Product Overview: Recombinant Mouse IL-15 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Description: Interleukin (IL)-15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity and has structural similarity to IL-2 that is secreted by mononuclear phagocytes following infection by viruses. Stimulation by IL-15 requires interaction of Il-15 with components of IL-2R, including IL-2Rbeta and probably IL-2R gamma, but not IL-2R alpha. This cytokine is produced by a wide variety of cells and tissues and like IL-2, stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
• Concentration: 0.5 mg/ml
• Form: Supplied as a liquid in PBS, pH7.4, containing 10% glycerol.
• Purity: > 90% by SDS - PAGE
• Sequence: 49-162 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
• Molecular Mass: 17.6 kDa (152aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Endotoxin Levels: < 1.0 EU per ug of protein (determined by LAL method)
• Bioactivity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1 ng/ml.
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: Il15 interleukin 15 [ Mus musculus ]
• Official Symbol: Il15
• Synonyms: Il15; interleukin 15; AI503618
• Gene ID: 16168
• mRNA Refseq: NM_008357
• Protein Refseq: NP_032383
• UniProt ID: P48346
• Chromosome Location: 8 C2; 8 38.0 cM
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: cytokine activity; cytokine receptor binding