Active Recombinant Mouse Inhba protein, His-tagged

• Cat.No.: Inhba-4069M
• Product Overview: Recombinant Mouse Inhba protein(NP_032406.1)(Met1-Ser424), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Ser424
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized human ACVR2B at 10 μg/mL (100 μl/well) can bind biotinylated mouse INHBA-His, The EC50 of biotinylated mouse INHBA-His is 0.161 μg/mL.2. Immobilized mouse INHBA-his at 10 μg/mL (100 μl/well) can bind human Follistatin Protein, The EC50 of human Follistatin Protein is 0.39 μg/mL.3. Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is typically 0.8-4 μg/mL.
• Molecular Mass: The secreted recombinant mouse INHBA comprises 415 amino acids and has a calculated molecular mass of 46.5 kDa. As a result of glycosylation, the apparent molecular mass of rmINHBA is approximately 18 & 43 kDa corresponding to the mature inhibin beta A chain and the pro domain respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 88 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Inhba inhibin beta-A [ Mus musculus ]
• Official Symbol: Inhba
• Synonyms: INHBA; inhibin beta-A; inhibin beta A chain; activin beta-A chain
• Gene ID: 16323
• mRNA Refseq: NM_008380
• Protein Refseq: NP_032406