Active Recombinant Mouse Il4 Protein (HIs21-Ser140), C-His tagged, Animal-free, Carrier-free

Cat.No. : Il4-59M
Product Overview : Recombinant Mouse Il4 Protein (HIs21-Ser140) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
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Species : Mouse
Source : E.coli
Tag : His
Protein Length : HIs21-Ser140
Description : Interleukin-4 (IL-4) is a key cytokine produced by activated T cells, mast cells, basophils, neutrophils and eosinophils. IL-4 is critical for the development of Th2-mediated responses, which is related to allergy and asthma. It can also regulate B cell responses, including survival, cell proliferation and gene expression. IL-4 also plays fundamental role for B-cell stimulation, including induction of the IgE isotype switch.
Form : Lyophilized
Bio-activity : Measure by its ability to induce HT-2 cells proliferation. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant mouse IL-4 is approximately >1 × 10⁶ IU/mg.
Molecular Mass : The protein has a calculated MW of 14.5 kDa.
The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : >98% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name Il4 interleukin 4 [ Mus musculus (house mouse) ]
Official Symbol Il4
Synonyms IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1;
Gene ID 16189
mRNA Refseq NM_021283
Protein Refseq NP_067258
UniProt ID P07750

Not For Human Consumption!

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