Active Recombinant Mouse Il4 Protein, His-Tagged

Cat.No. : Il4-01M
Product Overview : Recombinant mouse Il4 Protein, His-tagged was expressed in E. coli cell
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Species : Mouse
Source : E.coli
Tag : His
Description : The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th2 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to Interleukin 13.
Form : Lyophilized powder
AA Sequence : MHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISC
TMNESKSTSLKDFLESLKSIMQMDYS with polyhistidine tag at the C-terminus
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Bio-activity : Measure by its ability to induce HT-2 cells proliferation. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-4 is approximately >1 x 10^6 IU/mg.
Purity : ≥98% as determined by SDS-PAGE and HPLC.
Purified by Ni-NTA chromatography.
Storage Buffer : The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Storage : Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes : Please use within one month after protein reconstitution.
Gene Name Il4 interleukin 4 [ Mus musculus (house mouse) ]
Official Symbol Il4
Synonyms Il-4; BSF-1
Gene ID 16189
mRNA Refseq NM_021283.2
Protein Refseq NP_067258.1
UniProt ID P07750

Not For Human Consumption!

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