Active Recombinant Mouse Il4

• Cat.No.: Il4-297M
• Product Overview: Recombinant Mouse Il4 was expressed in CHO Cells.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Description: Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
• Form: Lyophilized after extensive dialysis against PBS.
• Bio-activity: ED50< 2="" ng/ml,="" measured="" in="" a="" cell="" proliferation="" assay="" using="" murine="" ht-2="" cells,="" corresponding="" to="" a="" specific="" activity="" of="">5 x 105 units/mg.
• Molecular Mass: 15 kDa, observed by reducing SDS-PAGE.
• AA Sequence: His23-Ser140.
• Endotoxin: < 0.2="" eu/μg,="" determined="" by="" lal="">
• Purity: >95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/ml.
• Publications:
  Alterations in the development of a M2 phenotype in glial cells: rotenone in vitro model of Parkinson's disease (2017)
  Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson’s disease patients (2022)
  Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair (2020)
  LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy (2019)

Gene Information

• Gene Name: Il4 interleukin 4 [ Mus musculus ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1
• Gene ID: 16189
• mRNA Refseq: NM_021283
• Protein Refseq: NP_067258
• UniProt ID: P07750
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
• Function: cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding

Citation

Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair

Journal: Scientific Reports    PubMed ID: 32606391    Data: 2020/6/30

Authors: Neus Rabaneda-Lombarte, Lucas Blasco-Agell, Carme Solà

Article Snippet:Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.Cells were treated with 50 ng/mL IL4 for 24 h in the absence or presence of MPP+ or rotenone.. Recombinant mouse IL4 expressed in CHO cells was used (Creative BioMart, Shirley, NY, USA).. A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.A stock solution of 50 mg/mL IL4 in a mixture of milli-Q H 2 O:culture medium (1:1) was prepared and stored at – 20 °C.

Effect of MPP+ treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 10 or 25 μM MPP+, in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means + SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. & p < 0.05, && p < 0.01 and &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance for the effects of MPP+ alone.

Effect of rotenone treatment on the mRNA expression of anti-inflammatory markers. IL10, TGFβ, IL1ra, Arg1, MR, Fizz1 and Ym1 mRNA expression in primary ( a ) mixed glial cultures and ( b ) microglial cultures treated for 24 h with 40 or 100 nM rotenone (Rot), in the absence and presence of IL4 (50 ng/mL). Genes encoding Rn18s and β-actin were used as the reference genes. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); # p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test. &&& p < 0.001 vs C, one-way ANOVA and Newman-Keuls post-hoc test considering only the IL4-free groups, which was performed to detect whether the high values observed in the IL4 group hindered the detection of a statistical significance of the effects of Rot alone.

ARG1 and MR protein levels in primary glial cell cultures treated with MPP+ or rotenone. ARG1 and MR levels were determined in the total protein extracts from ( a , c ) mixed glial cultures and ( b , d ) microglial cultures treated with 10 or 25 μM MPP+ or 40 or 100 nM rotenone (Rot) for 24 h, in the absence or presence of IL4 (50 ng/mL). Images of representative western blots are presented. Bars correspond to the means+ SEM of four independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs control (C); #p < 0.05, ## p < 0.01 and ### p < 0.001 vs IL4; one-way ANOVA and Newman-Keuls post-hoc test.