Active Recombinant Mouse IL3 protein, His-tagged

• Cat.No.: IL3-0221M
• Product Overview: Recombinant Mouse IL3 protein(Ala27-Cys166), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 27-166 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse IL-3 R alpha, His Tag on CM5 Chip can bind Mouse IL-3 Protein, His Tag with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala27-Cys166
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il3 interleukin 3 [ Mus musculus ]
• Official Symbol: IL3
• Synonyms: IL3; interleukin 3; interleukin-3; mast cell growth factor; P-cell-stimulating factor; hematopoietic growth factor; multipotential colony-stimulating factor; BPA; PSF; HCGF; Il-3; MCGF; Csfmu
• Gene ID: 16187
• mRNA Refseq: NM_010556
• Protein Refseq: NP_034686