Active Recombinant Mouse IL25 protein, His-tagged

• Cat.No.: IL25-2161M
• Product Overview: Recombinant Mouse IL25 protein(NP_542767.1)(Val 17-Ala 169), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Val 17-Ala 169
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized IL17RB Protein, Mouse, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind IL25/IL17E Protein, Mouse, Recombinant (His Tag), the EC50 is 4-20 ng/mL.2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 4-14ng/mL.
• Molecular Mass: The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il25 interleukin 25 [ Mus musculus ]
• Official Symbol: IL25
• Synonyms: IL25; interleukin 25; interleukin-25; interleukin 17E; Il17e; IL-17e
• Gene ID: 140806
• mRNA Refseq: NM_080729
• Protein Refseq: NP_542767