Active Recombinant Mouse Il1a Protein

• Cat.No.: Il1a-098M
• Product Overview: Recombinant protein of mouse interleukin-1a/IL-1a (Ser115-Ser270) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 115-270 a.a.
• Description: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Bio-activity: ED50 is less than 0.01 ng/ml as determined by the dose-dependent stimulation of mouse D10S cells.
  Specific Activity of 1.0 x 108 IU/mg.
• Molecular Mass: 18 kDa
• Endotoxin: < 0.1 EU per µg protein as determined by LAL test
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 50mM Tris-HCl, 200mM NaCl, pH 8.0.
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]
• Official Symbol: Il1a
• Synonyms: Il1a; interleukin 1 alpha; Il-1a; interleukin-1 alpha; IL-1 alpha
• Gene ID: 16175
• mRNA Refseq: NM_010554
• Protein Refseq: NP_034684
• UniProt ID: P01582