Active Recombinant Mouse Il19 Protein (153 aa)

• Cat.No.: Il19-367I
• Product Overview: Recombinant mouse Interleukin-19 (rmIL-19) produced in E. coli is a single non-glycosylated polypeptide chain containing of153 amino acids. A fully biologically active molecule, rmIL-19 has a molecular mass of 17.7 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 153
• Description: Interleukin-19 (IL-19) is a cytokine belonging to the interleukin family. Structurally, IL-19 is grouped into the IL-10 sub-family, which also includes IL-20, IL-22, IL-24, and IL-26. In contrast to IL-10, which exists as a homodimer, IL-19 is stable and active as a monomer in vivo. IL-19 functions through the receptor complex composed of IL-20 Receptor α and IL-20 Receptor β, which is also utilized by IL-20 and IL-24. IL-19 is produced by active monocytes and stimulated synergistically by IL-17 and IL-13. The functions of IL-19 are to promote the development and function of Th2 cells and to enhance the production of Th2 cytokines. IL-19 is implicated in aging, vascular disease, Type I diabetes, and rheumatoid arthritis.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 50 ng/mL, measured by a cell proliferation assay using MCF-7 cells, corresponding to a specific activity of > 2 × 10^4 units/mg.
• Molecular Mass: 17.7 kDa, observed by non-reducing SDS-PAGE.
• AA Sequence: MLRRCLISVDMRLIEKSFHEIKRAMQTKDTFKNVTILSLENLRSIKPGDVCCMTNNLLTFYRDRVFQDHQERSLEVLRRISSIANSFLCVQKSLERCQVHRQCNCSQEATNATRIIHDNYNQLEVSSAALKSLGELNILLAWIDRNHLETPAA
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% by SDS-PAGE analysis.
• Storage: Lyophilized recombinant mouse Interleukin-19 (rmIL-19) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rmIL-19 remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against 50mM acetic acid.
• Reconstitution: Reconstituted in 50 mM acetic acid at 50 μg/mL.

Gene Information

• Gene Name: Il19 interleukin 19 [ Mus musculus ]
• Official Symbol: Il19
• Synonyms: IL19; interleukin 19; interleukin-19; IL-19
• Gene ID: 329244
• mRNA Refseq: NM_001009940
• Protein Refseq: NP_001009940
• UniProt ID: Q8CJ70