Active Recombinant Mouse IL12RB1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL12RB1-0225M
• Product Overview: Biotinylated Recombinant Mouse IL12RB1 protein(Gln20-Ser565), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-565 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Mouse IL-12 R beta 1, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human IL-12B, Fc Tag with a linear range of 0.4-13 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 64.7 kDa. The protein migrates as 93-103 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln20-Ser565
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il12rb1 interleukin 12 receptor, beta 1 [ Mus musculus ]
• Official Symbol: IL12RB1
• Synonyms: IL12RB1; interleukin 12 receptor, beta 1; interleukin-12 receptor subunit beta-1; IL-12R-beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; Interleukin-12 receptor beta-1 chain precursor (IL-12R-beta1) (Interleukin-12 receptor beta) (IL-12 receptor beta component); CD212; IL-12R[b]
• Gene ID: 16161
• mRNA Refseq: NM_008353
• Protein Refseq: NP_032379