Active Recombinant Mouse Ifnb1 Protein (Ile22-Asn182), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Ifnb1-41M
• Product Overview: Recombinant Mouse Ifnb1 Protein (Ile22-Asn182) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interferon Beta 1a (IFN beta 1a) is a leukocyte interferon, which is a variant of Interferon beta. IFN beta 1a is a 17.87 kDa protein containing 161 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to protect HeLa cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 5 pg/mL. The specific activity of recombinant mouse IFN beta 1a is > 1 x 10^9 IU/mg.
• Molecular Mass: The protein has a calculated MW of 20.68 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ile22-Asn182
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Ifnb1 interferon beta 1, fibroblast [ Mus musculus (house mouse) ]
• Official Symbol: Ifnb1
• Synonyms: IFNB1; interferon beta 1, fibroblast; interferon beta; Ifb; IFNB; IFN-beta
• Gene ID: 15977
• mRNA Refseq: NM_010510
• Protein Refseq: NP_034640
• UniProt ID: P01575