Active Recombinant Mouse Icam1 protein(Met1-Asn485), His&hFc-tagged

• Cat.No.: Icam1-3323M
• Product Overview: Recombinant Mouse ICAM1 (NP_034623.1) (Met 1-Asn 485) was expressed in HEK293, fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His&hFc
• Protein length: Met1-Asn485
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM1 coated plates (12.5 μg/ml, 100 μl/well), approximately >50% cells will adhere specifically .
• Molecular Mass: The recombinant mouse ICAM1/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 706 amino acids and has a predicted molecular mass of 78.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm ICAM1/Fc monomer is approximately 120-130 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icam1 intercellular adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Icam1
• Synonyms: ICAM1; intercellular adhesion molecule 1; myD10; CD54; Ly-47; Icam-1; MALA-2; MGC6195
• Gene ID: 15894
• mRNA Refseq: NM_010493
• Protein Refseq: NP_034623