Active Recombinant Mouse Ibsp Protein, His-tagged

• Cat.No.: Ibsp-600M
• Product Overview: Recombinant Mouse Ibsp (Accession # NP_032344) Met1-Gln324, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Phe17
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x 10^4 cells/well are added to recombinant mouse IBSP coated plates (3 µg/mL with 100 µL/well), approximately 60 - 80% will adhere after 30 minutes at 37 °C.Optimal concentration depends on cell type as well as the application or research objectives.
• Molecular Mass: Recombinant Mouse Ibsp, His-tagged has a calculated MW of 34.9 kDa. In SDS-PAGE migrates as 70-90 kDa, reducing conditions.
• Protein length: Met1-Gln324
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Ibsp integrin binding sialoprotein [ Mus musculus ]
• Official Symbol: Ibsp
• Synonyms: IBSP; integrin binding sialoprotein; bone sialoprotein 2; bone sialoprotein II; cell-binding sialoprotein; integrin-binding sialoprotein; BSP; Bsp2; BSPII; BSP II
• Gene ID: 15891
• mRNA Refseq: NM_008318
• Protein Refseq: NP_032344