Active Recombinant Mouse Hpgd protein, His-tagged

• Cat.No.: Hpgd-2525M
• Product Overview: Recombinant Mouse Hpgd(Met 1-Ser 269) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Description: Mouse 15-hydroxyprostaglandin dehydrogenase [NAD+], also known as Prostaglandin dehydrogenase 1, HPGD, and PGDH1, is a member of the short-chain dehydrogenases/reductases (SDR) family. Prostaglandins (PGs) play a key role in the onset of labor in many species and regulate uterine contractility and cervical dilatation. Therefore, the regulation of prostaglandin output by PG synthesizing and metabolizing enzymes in the human myometrium may determine uterine activity patterns in human labor both at preterm and at term. Prostaglandin dehydrogenase (PGDH) metabolizes prostaglandins (PGs) to render them inactive. HPGD is down-regulated by cortisol, dexamethasone and betamethasone and down-regulated in colon cancer. It is up-regulated by TGFB1. HPGD contributes to the regulation of events that are under the control of prostaglandin levels. HPGD catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. and inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) , cranioosteoarthropathy (COA), and isolated congenital nail clubbing.
• Source: E. coli
• Species: Mouse
• Tag: His
• Predicted N Terminal: Met 1
• Form: Lyophilized from sterile PBS, pH 8.0, 20% glycerol1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Bio-activity: Measured by its ability to bind Rhesus ErbB3-His in functional Elisa.
• Molecular Mass: The recombinant mouse HPGD consisting of 279 amino acids and has a calculated molecular mass of 30.6 kDa. rmHPGD migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions as predicted.
• Protein length: Met 1-Ser 269
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Hpgd hydroxyprostaglandin dehydrogenase 15 (NAD) [ Mus musculus ]
• Official Symbol: Hpgd
• Synonyms: HPGD; hydroxyprostaglandin dehydrogenase 15 (NAD); 15-hydroxyprostaglandin dehydrogenase [NAD(+)]; PGDH; prostaglandin dehydrogenase 1; 15-hydroxyprostaglandin dehydrogenase; 15-PGDH; AV026552; MGC14001
• Gene ID: 15446
• mRNA Refseq: NM_008278
• Protein Refseq: NP_032304
• UniProt ID: Q8VCC1
• Chromosome Location: 8; 8 B3.2
• Pathway: Arachidonic acid metabolism via COX (Cyclooxygenase) pathway, organism-specific biosystem; Arachidonic acid metabolism via LOX (Lipooxygenase) pathway, organism-specific biosystem; Prostaglandin Synthesis and Regulation, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem; biosynthesis of prostaglandins, organism-specific biosystem
• Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; NAD binding; NAD+ binding; catalytic activity; nucleotide binding; oxidoreductase activity; prostaglandin E receptor activity