Active Recombinant Mouse Hepatocyte Growth Factor, His-tagged

• Cat.No.: Hgf-728M
• Product Overview: Recombinant Mouse Hgfprotein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Met1-Ser653. The proform was purified, activated and further purified.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-653 a.a.
• Description: Hgf is a serineendopeptidase that cleaves at the peptide bond between Arg494 and Val495 ofsingle-chain human Hgf precursor, generating the active heterodimer. Hgf isproduced and secreted by the liver and normally circulates in the blood as aninactive zymogen. The zymogen has a weak affinity for heparin but acquires astrong affinity for heparin upon activation that is linked to bloodcoagulation.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and CaCl2.
• N-terminalSequence: Val370 & Ile406
• Molecular Weight: 32 kDa & 28 kDa
• Activity: Measuredby its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 100 pmoles/min/μg, as measured underthe described conditions.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 35 kDaand 32 kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months fromdate of receipt, -70°C as supplied. 3 months, -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: Hgf hepatocyte growth factor [ Musmusculus ]
• Official Symbol: Hgf
• Synonyms: Hgf; hepatocytegrowth factor; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; SF; scatter factor;hepatopoeitin-A
• Gene ID: 15234
• mRNA Refseq: NM_010427
• Protein Refseq: NP_034557
• UniProt ID: Q08048
• Chromosome Location: 5 A2-A3; 5 4.0 cM
• Pathway: Cytokine-cytokinereceptor interaction; Focal Adhesion; Focal adhesion; Hemostasis; Malaria;Melanoma; Pathways in cancer; Platelet activation; Platelet degranulation;Renal cell carcinoma; Response to elevated platelet cytosolic Ca2+;Signaling of Hepatocyte Growth Factor Receptor
• Function: catalytic activity;growth factor activity; protein heterodimerization activity; serine-typeendopeptidase activity