Active Recombinant Mouse Gzmb Protein

• Cat.No.: Gzmb-070M
• Product Overview: Purified recombinant protein of Mouse granzyme B (Gzmb) without tag was expressed Sf9 cell.

Specification

• Species: Mouse
• Source: Insect Cells
• Description: This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis.
• Bio-activity: Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25C, is greater than 750 nM/min per ug of enzyme.
• Molecular Mass: 28.9 kDa
• AA Sequence: IIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Gzmb granzyme B [ Mus musculus (house mouse) ]
• Official Symbol: Gzmb
• Synonyms: Gzmb; granzyme B; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11; granzyme B(G,H); cytotoxic cell protease 1; fragmentin-2; EC 3.4.21.79
• Gene ID: 14939
• mRNA Refseq: NM_013542
• Protein Refseq: NP_038570
• UniProt ID: P04187